最后一次
If you say that it is the last time you will do something, you mean that you will never do it again.
He never even thanked me, so that's the last time I do him a favour. 他甚至从未向我表示过感谢，所以那是我最后一次帮他。
That's the last time we're inviting our neighbours to a party - they didn't talk to anyone!
It's the last time I'm going to that restaurant. The food was terrible.
She decided that was the last time she'd lend him money.
I can't believe you let them criticize me like that. It's the last time I'm standing up for you, I can tell you.
See also: last adjective adverb pronoun noun